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human shhn  (Biogen Inc)

 
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    Biogen Inc human shhn
    Human Shhn, supplied by Biogen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human shhn/product/Biogen Inc
    Average 90 stars, based on 1 article reviews
    human shhn - by Bioz Stars, 2026-04
    90/100 stars

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    Effect of recombinant ShhN treatment on Gli1 mRNA expression in pancreatic CAFs. (a) Gli1 mRNA levels were assessed 24 hours after treatment of CAF27 cells with indicated concentrations of recombinant ShhN. (b) Comparison of ShhN treatment in control pancreatic fibroblasts and pancreatic CAFs. Gli1 mRNA levels were assessed 24 hours after treatment of HPNE, SC2, or CAF cells with indicated concentrations of recombinant ShhN. Relative Gli1 mRNA levels after normalization to the corresponding 18S rRNA levels are shown. Data are means of four independent experiments; bars are SD values.

    Journal:

    Article Title: Overexpression of Smoothened activates the Sonic Hedgehog signaling pathway in pancreatic cancer associated fibroblasts

    doi: 10.1158/1078-0432.CCR-09-1913

    Figure Lengend Snippet: Effect of recombinant ShhN treatment on Gli1 mRNA expression in pancreatic CAFs. (a) Gli1 mRNA levels were assessed 24 hours after treatment of CAF27 cells with indicated concentrations of recombinant ShhN. (b) Comparison of ShhN treatment in control pancreatic fibroblasts and pancreatic CAFs. Gli1 mRNA levels were assessed 24 hours after treatment of HPNE, SC2, or CAF cells with indicated concentrations of recombinant ShhN. Relative Gli1 mRNA levels after normalization to the corresponding 18S rRNA levels are shown. Data are means of four independent experiments; bars are SD values.

    Article Snippet: The cells were incubated for 24 hours in low-serum medium (DMEM + 0.5% FBS) containing 0, 0.5, 1.0, or 2.5 μg/mL recombinant human sonic hedgehog (ShhN) ligand (1845-SH, R&D Systems, Inc.).

    Techniques: Recombinant, Expressing, Comparison, Control

    Effects of Smo siRNA and recombinant ShhN treatment on Gli1 mRNA expression in pancreatic CAFs. (a) Smo and (b) Gli1 levels were assessed by qRT-PCR in CAF26 cells transfected with 2μM non-targeting or Smo siRNA for 72 hours. (c) CAF27 cells were transfected with 2μM non-targeting or Smo siRNA and treated with the indicated concentrations of recombinant ShhN 48 hours later. Gli1 mRNA levels were assessed at 72 hours post-transfection. Relative Smo or Gli1 mRNA levels after normalization to the corresponding 18S rRNA levels are shown. Data represent the mean of three independent experiments; error bars are SD values.

    Journal:

    Article Title: Overexpression of Smoothened activates the Sonic Hedgehog signaling pathway in pancreatic cancer associated fibroblasts

    doi: 10.1158/1078-0432.CCR-09-1913

    Figure Lengend Snippet: Effects of Smo siRNA and recombinant ShhN treatment on Gli1 mRNA expression in pancreatic CAFs. (a) Smo and (b) Gli1 levels were assessed by qRT-PCR in CAF26 cells transfected with 2μM non-targeting or Smo siRNA for 72 hours. (c) CAF27 cells were transfected with 2μM non-targeting or Smo siRNA and treated with the indicated concentrations of recombinant ShhN 48 hours later. Gli1 mRNA levels were assessed at 72 hours post-transfection. Relative Smo or Gli1 mRNA levels after normalization to the corresponding 18S rRNA levels are shown. Data represent the mean of three independent experiments; error bars are SD values.

    Article Snippet: The cells were incubated for 24 hours in low-serum medium (DMEM + 0.5% FBS) containing 0, 0.5, 1.0, or 2.5 μg/mL recombinant human sonic hedgehog (ShhN) ligand (1845-SH, R&D Systems, Inc.).

    Techniques: Recombinant, Expressing, Quantitative RT-PCR, Transfection